Qubit dsDNA HS Assay Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Do not label the side of the tube as this could interfere with the sample read.

Protocol tips
Do not label the side of the tube as this could interfere with the sample read.

Publication protocol

Massive parallel sequencing
qPCR-qualified ChIP assays were quantified (Qubit dsDNA HS assay kit, Invitrogen); 3–10 ng of the ChIP material was used for preparing Illumina sequencing libraries following a multiplexing approach (NEXTflexTM ChIP-seq Bioo Scientific, reference 5143-02). Prepared sequencing libraries were sequenced on an Illumina HiSeq2000 instrument. Regular Illumina pipelines were used for image processing and base calling. Sequence files were then aligned to the human genome assembly using default parameters (hg19; Bowtie).

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Qubit dsDNA HS Assay Kit below.

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