Wizard® Genomic DNA Purification Kit

Protocol tips

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	For blood DNA isolation, blood must be collected in EDTA, heparin or citrate anticoagulant tubes to prevent clotting.

Protocol tips
For blood DNA isolation, blood must be collected in EDTA, heparin or citrate anticoagulant tubes to prevent clotting.

Publication protocol

2.2. Preparation of Genomic DNA
The stored isolates were grown overnight on chocolate agar plates (BD Diagnostics, Sparks, MD) at 37°C with 5% CO2 in a humid environment. Genomic DNA was isolated using the Wizard genomic DNA purification kit (Promega, Madison, WI.) according to the manufacturer’s instructions and resuspended in 1× Tris-EDTA buffer (10 mM Tris-HCL and 1mM EDTA at pH 8). The majority of the DNA was kept at −20°C for storage, with a small aliquot stored at 4°C for use.

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Manufacturer protocol

Download the product protocol from Promega for Wizard® Genomic DNA Purification Kit below.

Download manufacturer protocol

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