Wizard® Genomic DNA Purification Kit

DNA isolation / purification Bacteria - Gram negative Haemophilus influenzae

Experiment
DNA isolation / purification Bacteria - Gram negative Haemophilus influenzae
Product
Wizard® Genomic DNA Purification Kit from Promega
Manufacturer
Promega

Protocol tips

Protocol tips
For blood DNA isolation, blood must be collected in EDTA, heparin or citrate anticoagulant tubes to prevent clotting.

Publication protocol

2.2. Preparation of Genomic DNA
The stored isolates were grown overnight on chocolate agar plates (BD Diagnostics, Sparks, MD) at 37°C with 5% CO2 in a humid environment. Genomic DNA was isolated using the Wizard genomic DNA purification kit (Promega, Madison, WI.) according to the manufacturer’s instructions and resuspended in 1× Tris-EDTA buffer (10 mM Tris-HCL and 1mM EDTA at pH 8). The majority of the DNA was kept at −20°C for storage, with a small aliquot stored at 4°C for use.

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Reviews

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Discussion

Discussion

5 years ago

Author: Israel

How can I improve my DNA yield?

The DNA concentration after using this DNA isolation kit is sometimes too low and thus it is not sufficient for my follow-up experiments. How can I improve it?

Discussion

5 years ago

Author: Milena Alexeyeva Russian Federation

Tips on storing DNA templates?

Hello there! I just started doing experiments on bacterial DNA and I would like your opinion on storing DNA templates. Which are the desired and most optimal conditions?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Bacteria - Gram negative Haemophilus influenzae using Wizard® Genomic DNA Purification Kit from Promega.

Paper title
Population Structure in Nontypeable
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Manufacturer protocol

Download the product protocol from Promega for Wizard® Genomic DNA Purification Kit below.

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Videos

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