QIAamp DNA Mini Kit

DNA isolation / purification Bacteria - Gram negative Haemophilus influenzae

Experiment
DNA isolation / purification Bacteria - Gram negative Haemophilus influenzae
Product
QIAamp DNA Mini Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Downstream tips

- Include RNAse treatment for 15-20 min.
- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C
- Use prewarmed TE buffer to elute the DNA

Publication protocol

2.3. DNA Extraction
The DNA was extracted from reference bacterial strains and clinical isolates using a QIAamp DNA Mini Kit (Qiagen, Germany), according to the manufacturer's instruction. The DNA concentration and purity were measured using a spectrophotometer (Eppendorf Biophotometer, Germany), while the integrity of the extracted DNA was tested using a FluoroSafe-stained agarose gel. Purified DNA was kept at −20°C until it was used.

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Reviews

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Discussion

Discussion

5 years ago

Author: Israel

How can I improve my DNA yield?

The DNA concentration after using this DNA isolation kit is sometimes too low and thus it is not sufficient for my follow-up experiments. How can I improve it?

Discussion

5 years ago

Author: Milena Alexeyeva Russian Federation

Tips on storing DNA templates?

Hello there! I just started doing experiments on bacterial DNA and I would like your opinion on storing DNA templates. Which are the desired and most optimal conditions?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Bacteria - Gram negative Haemophilus influenzae using QIAamp DNA Mini Kit from Qiagen.

Paper title
A Thermostabilized, One-Step PCR Assay for Simultaneous Detection of and
Full paper
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Manufacturer protocol

Download the product protocol from Qiagen for QIAamp DNA Mini Kit below.

Download PDF Download manufacturer protocol

Videos

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