Bone DNA Purification Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
		- Include RNAse treatment for 15-20 min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C - Use prewarmed TE buffer to elute the DNA

Downstream tips
- Include RNAse treatment for 15-20 min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C - Use prewarmed TE buffer to elute the DNA

Publication protocol

DNA extraction was performed in another pre-PCR room. For initial decalcification, 1 mL of 0.5 M EDTA (pH 8.0) was added to the tooth powder and incubated at 30 °C under rotation for 1 h. The decalcified material was incubated overnight at 56 °C using the lysis buffer of the Gene Matrix Bone DNA Purification Kit (EURx, Gdansk, Poland). DNA extraction was performed following the manufacturer’s recommendations. Final DNA elution was executed in 50 µL and 30 µL elution buffer, for the first and second DNA extraction, respectively. DNA concentration was measured using the Qubit dsDNA HS Assay Kit with the Qubit 3.0 fluoremeter (Thermo Fisher Scientific, Waltham, MA, USA).

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Manufacturer protocol

Download the product protocol from Eurx for Bone DNA Purification Kit below.

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