Bone DNA Purification Kit

DNA isolation / purification Tissue - bone

Experiment
DNA isolation / purification Tissue - bone
Product
Bone DNA Purification Kit from Eurx
Manufacturer
Eurx

Protocol tips

Downstream tips
- Include RNAse treatment for 15-20 min.
- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C
- Use prewarmed TE buffer to elute the DNA

Publication protocol

DNA extraction was performed in another pre-PCR room. For initial decalcification, 1 mL of 0.5 M EDTA (pH 8.0) was added to the tooth powder and incubated at 30 °C under rotation for 1 h. The decalcified material was incubated overnight at 56 °C using the lysis buffer of the Gene Matrix Bone DNA Purification Kit (EURx, Gdansk, Poland). DNA extraction was performed following the manufacturer’s recommendations. Final DNA elution was executed in 50 µL and 30 µL elution buffer, for the first and second DNA extraction, respectively. DNA concentration was measured using the Qubit dsDNA HS Assay Kit with the Qubit 3.0 fluoremeter (Thermo Fisher Scientific, Waltham, MA, USA).

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Discussion

Discussion

5 years ago

Author: Denmark

What DNA isolation kit would work for insect samples?

Hello everyone! I am currently using different DNA isolation kits to extract DNA from insects. Even though I am able to successfully extract DNA I would like to maximize the yield. Do you have any tips that might help me with that even if the kits are not specifically designed for insect samples?

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Papers

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Manufacturer protocol

Download the product protocol from Eurx for Bone DNA Purification Kit below.

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Videos

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