QIAamp DNA FFPE Tissue Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
		- Include RNAse treatment for 15-20 min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C - Use prewarmed TE buffer to elute the DNA

Downstream tips
- Include RNAse treatment for 15-20 min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C - Use prewarmed TE buffer to elute the DNA

Publication protocol

Selection of Patients and Samples and DNA Extraction
Institutional ethical guidelines were followed for this retrospective study. Between 2000 and 2012, a total of 91 cases of fibrous dysplasia were diagnosed histologically in the University Hospital of Tours (France), including 43 cases of fibrous dysplasia referred by other centers for second diagnosis opinion. Only two patients had a polyostotic form of fibrous dysplasia and the others had monostotic fibrous dysplasia. To select the most representative formalin-fixed and paraffin-embedded tissues, hematoxylin–eosin and saffron slides were reviewed by two pathologists (GdP and FTD) before the molecular studies. Genomic DNA (gDNA) was extracted using the QIAamp DNA formalin-fixed and paraffin-embedded extraction kit (QIAGEN) from 10-μm sections cut from tissue blocks in 81 cases and from snap-frozen tissue (stored at −80°C) in 18 cases, using the QIAamp DNA Mini Kit (QIAGEN). Tissue blocks and snap-frozen samples were available for eight tumors. Thick sections of formalin-fixed and paraffin-embedded tissue were dewaxed by extraction in 100% xylene and washed with 100% ethanol. The samples were air-dried before DNA extraction. The extracted DNA was quantified using a Spectrophotometer

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Manufacturer protocol

Download the product protocol from Qiagen for QIAamp DNA FFPE Tissue Kit below.

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