cobas® DNA Sample Preparation Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
All solutions stored at 15 - 30°C should be clear. If precipitate is present in any reagent, warm the solution to 37°C until the precipitate dissolves. Do not use until all precipitate has been dissolved.		- Include RNAse treatment for 15-20 min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C - Use prewarmed TE buffer to elute the DNA

Upstream tips
All solutions stored at 15 - 30°C should be clear. If precipitate is present in any reagent, warm the solution to 37°C until the precipitate dissolves. Do not use until all precipitate has been dissolved.
Downstream tips
- Include RNAse treatment for 15-20 min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C - Use prewarmed TE buffer to elute the DNA

Publication protocol

Cobas sample preparation kit isolation method
The whole experiment was also performed according
to Cobas sample preparation kit (Roche Molecular
Systems, Branchburg, NJ, USA, ‘Cobas method’)
manufacturer’s instruction. Except the reagents used were different, the main steps are the same as QIAamp method.
Moreover, while resuspend the tissue pellet in 180 μL
DNA Tissue Lysis Buffer, we added 70 μL of reconstituted proteinase K, and in the last step, 100 μL DNA EB (elution buffer) was added to the center of each membrane, after incubating the elution tube at 15°C to 30°C for 5 min. The elution tube was centrifuged at 8000×g for 1 min to collect eluate into the elution tube. During the whole process, we did not add any RNase A.

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Manufacturer protocol

Download the product protocol from Roche Lifesciences for cobas® DNA Sample Preparation Kit below.

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