TIANamp Genomic DNA Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
		- Include RNAse treatment for 15-20 min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C - Use prewarmed TE buffer to elute the DNA

Downstream tips
- Include RNAse treatment for 15-20 min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C - Use prewarmed TE buffer to elute the DNA

Publication protocol

Real-Time qPCR Analysis
Total RNAs were obtained from the indicated samples using TRIzol reagent (Invitrogen, 15596-018), referring to the instruction manual. Then, the total RNAs were reverse-transcribed to complementary DNA by the FastQuant RT kit (with genomic deoxyribonuclease [gDNase]) (TIANGEN, KR106-02) following the manufacturer’s recommendations. Real-time qPCR was performed to quantify the mRNA expression level in the indicated samples. Each RNA sample, in triplicate, was quantified by SuperReal PreMix Plus (SYBR Green) (TIANGEN, FP205-02) according to the user manual. Signals were visualized by ABI7500 real-time PCR System (Applied Biosystems). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was employed as an endogenous control. All primers used were designed by the software or from the relative references, and the sequences are listed in Table S3. Fold changes of relative gene expression levels were computed by the comparative Ct method (fold change = 2−ΔΔCt).

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Manufacturer protocol

Download the product protocol from Tiangen for TIANamp Genomic DNA Kit below.

Download manufacturer protocol

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