Wizard® Genomic DNA Purification Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	For blood DNA isolation, blood must be collected in EDTA, heparin or citrate anticoagulant tubes to prevent clotting.

Protocol tips
For blood DNA isolation, blood must be collected in EDTA, heparin or citrate anticoagulant tubes to prevent clotting.

Publication protocol

DNA isolation and quantification
Total DNA from primary astrocytes was prepared using Wizard Genomic DNA Purification Kit (Promega Corporation, Madison, WI, USA) and following the manufacturer's instructions. The relative copy numbers of mitochondrial and nuclear DNA were determined by real-time PCR with primers specific to the COX3 (mitochondrial) and 18SrDNA (nuclear) genes, 100 ng DNA, and SYBRGreen PCR master mix (Bio-Rad, Hercules, CA, USA) on an iCycler real-time PCR machine (Bio-Rad).

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Cells - Primary cells Rat astrocytes using Wizard® Genomic DNA Purification Kit from Promega.

Manufacturer protocol

Download the product protocol from Promega for Wizard® Genomic DNA Purification Kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing DNA isolation / purification Cells - Primary cells Rat astrocytes using Wizard® Genomic DNA Purification Kit from Promega. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.