|Add ß-mercaptoethanol to the solution and homogenize 3–4 times for each 20 s using a commercial homogenizer (e.g., Polytron, Dounce).
Alternatively, other homogenization tools like mortar and pestle in the presence of liquid nitrogen may be used. In order to reduce the viscosity pass the lysate 3 times through a sterile plastic syringe fitted with a 20 gauge needle.
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4 years ago
Author: R. Verma
During centrifugation, the column got clogged and I was unable to continue with the protocol. How can I unclog it?
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