Gentra Puregene Yeast/Bact. Kit

DNA isolation / purification Bacteria - Gram positive Pseudomonas

Experiment
DNA isolation / purification Bacteria - Gram positive Pseudomonas
Product
Gentra Puregene Yeast/Bact. Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Upstream tips
Frozen yeast/bacterial samples should be thawed and equilibrated to room temperature (15–25°C) before beginning the procedure.

Publication protocol

Bacterial genomic DNA was extracted from 1·5 mL broth cultures by the use of a Puregene DNA isolation kit (Gentra System‐Flowgen) following the manufacturer's instructions; the DNA eluate was resuspended in 150–200 μL elution buffer. DNA was extracted from 500 to 1000 μL crude plant extract (samples with symptoms) and concentrated plant extract (symptomless sample) using a DNeasy Plant Mini Kit (QIAGEN), according to the manufacturer's instructions. DNA concentrations were determined using a NanoDrop ND‐1000 spectrophotometer (Thermo Fisher Scientific), then diluted in sterile milli‐Q water to a final concentration of 5 ng μL−1 and stored in aliquots at −20°C.

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Reviews

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Discussion

Discussion

5 years ago

Author: Israel

How can I improve my DNA yield?

The DNA concentration after using this DNA isolation kit is sometimes too low and thus it is not sufficient for my follow-up experiments. How can I improve it?

Discussion

5 years ago

Author: Milena Alexeyeva Russian Federation

Tips on storing DNA templates?

Hello there! I just started doing experiments on bacterial DNA and I would like your opinion on storing DNA templates. Which are the desired and most optimal conditions?

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Papers

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Manufacturer protocol

Download the product protocol from Qiagen for Gentra Puregene Yeast/Bact. Kit below.

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