ROS-Glo™ H2O2 Assay

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- It is recommended to keep the final concentration of solvents such as DMSO to ≤1%		- Lower signals than expected when H2 O2 is applied to cells is due to catalase and glutathione peroxidase, that can rapidly eliminate H2O2

Upstream tips
- It is recommended to keep the final concentration of solvents such as DMSO to ≤1%
Downstream tips
- Lower signals than expected when H2 O2 is applied to cells is due to catalase and glutathione peroxidase, that can rapidly eliminate H2O2

Publication protocol

Cellular ROS were measured using a commercial kit (ROS-GloTM H2O2 Assay, Promega) according to the manufacturer’s instructions.

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Manufacturer protocol

Download the product protocol from Promega for ROS-Glo™ H2O2 Assay below.

Download manufacturer protocol

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