ROS-Glo™ H2O2 Assay

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- It is recommended to keep the final concentration of solvents such as DMSO to ≤1%.

Upstream tips
- It is recommended to keep the final concentration of solvents such as DMSO to ≤1%.

Publication protocol

CellTiter-Glo (Promega, Madison, WI, USA) was used for cell viability assays (24 h after treatment) and ATP assays (at indicated time points during or after β-lap treatment). The following assays were purchased from Promega: GSH/GSSG-Glo, NAD/NADH-Glo, NADP/NADPH-Glo, and ROS-Glo and were used as directed. Unless otherwise noted, all raw luminescent values for treatment conditions were normalized to the signal from untreated cells (T/C). Standard curves were generated to ensure linearity.

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Papers

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Manufacturer protocol

Download the product protocol from Promega for ROS-Glo™ H2O2 Assay below.

Download manufacturer protocol

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