OxiSelect™ Intracellular ROS Assay Kit (Green Fluorescence)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- 10,000 cells were seeded in 96-well. - Cells were incubated with serum free medium fro 24h before treated with DCFDA.

Upstream tips
- 10,000 cells were seeded in 96-well. - Cells were incubated with serum free medium fro 24h before treated with DCFDA.

Publication protocol

Cells were seeded at a density of 10,000 in 96-well clear bottom black plates, allowed to equilibrate overnight, and starved in SFM for 24 hours followed by treatments. Intracelluar ROS levels were determined by using OxiSelect™ Intracellular ROS Assay Kit according to manufacturer's guideline (Cell Biolabs, Inc., San Diego, CA)

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing ROS assay cell type - MDA-MB-231 using OxiSelect™ Intracellular ROS Assay Kit (Green Fluorescence) from Cell Biolabs.

Manufacturer protocol

Download the product protocol from Cell Biolabs for OxiSelect™ Intracellular ROS Assay Kit (Green Fluorescence) below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing ROS assay cell type - MDA-MB-231 using OxiSelect™ Intracellular ROS Assay Kit (Green Fluorescence) from Cell Biolabs. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.