ROS-ID® Total ROS/Superoxide detection kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Cells should be healthy and not overcrowded since results of the experiments will depend significantly on the cells’ condition.	- Cells should be pre-treated with the ROS Inhibitor at least 30 minutes prior to induction.

Upstream tips
-Cells should be healthy and not overcrowded since results of the experiments will depend significantly on the cells’ condition.
Protocol tips
- Cells should be pre-treated with the ROS Inhibitor at least 30 minutes prior to induction.

Publication protocol

Reactive oxygen species were measured using a total ROS detection kit (Enzo Life Sciences) using a protocol modified from Hafer et al (2008). Samples were acquired on a FACScan flow cytometer.

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Manufacturer protocol

Download the product protocol from Enzo Life Sciences for ROS-ID® Total ROS/Superoxide detection kit below.

Download manufacturer protocol

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