OxiSelect™ In Vitro ROS/RNS Assay Kit (Green Fluorescence)

ROS assay cell type - T47D

Experiment
ROS assay cell type - T47D
Product
OxiSelect™ In Vitro ROS/RNS Assay Kit (Green Fluorescence) from Cell Biolabs
Manufacturer
Cell Biolabs

Protocol tips

Upstream tips
- 10,000 cells were seeded in a 96 well plate.

- Cells were starved with serum free medium for 24h before the assay

Publication protocol

Cells were seeded at a density of 10,000 in 96-well clear bottom black plates, allowed to equilibrate overnight, and starved in SFM for 24 hours followed by treatments. Intracelluar ROS levels were determined by using OxiSelect™ Intracellular ROS Assay Kit according to manufacturer's guideline (Cell Biolabs, Inc., San Diego, CA)

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Papers

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Paper title
IGF-I regulates redox status in breast cancer cells by activating the amino acid transport molecule xC
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Manufacturer protocol

Download the product protocol from Cell Biolabs for OxiSelect™ In Vitro ROS/RNS Assay Kit (Green Fluorescence) below.

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