OxiSelect™ In Vitro ROS/RNS Assay Kit (Green Fluorescence)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- 10,000 cells were seeded in a 96 well plate. - Cells were starved with serum free medium for 24h before the assay

Upstream tips
- 10,000 cells were seeded in a 96 well plate. - Cells were starved with serum free medium for 24h before the assay

Publication protocol

Cells were seeded at a density of 10,000 in 96-well clear bottom black plates, allowed to equilibrate overnight, and starved in SFM for 24 hours followed by treatments. Intracelluar ROS levels were determined by using OxiSelect™ Intracellular ROS Assay Kit according to manufacturer's guideline (Cell Biolabs, Inc., San Diego, CA)

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Manufacturer protocol

Download the product protocol from Cell Biolabs for OxiSelect™ In Vitro ROS/RNS Assay Kit (Green Fluorescence) below.

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