CM-H2DCFDA (General Oxidative Stress Indicator)

ROS assay cell type - T47D

Experiment
ROS assay cell type - T47D
Product
CM-H2DCFDA (General Oxidative Stress Indicator) from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Upstream tips
- Do not dissolve products until immediately prior to use
Protocol tips
- Cells were measure for ROS levels after 24h of platting.

Publication protocol

Intracellular ROS levels were measured at 24 hours post-plating using CM-H2DCFDA (Thermo Fisher Scientific) as described previously [31]. Each of 3 independent experiments included a negative control of unlabeled cells to verify staining specificity. Results were normalized to cell number obtained by staining with crystal violet.

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing ROS assay cell type - T47D using CM-H2DCFDA (General Oxidative Stress Indicator) from Thermo Fisher Scientific.

Paper title
Chronic Inhibition of STAT3/STAT5 in Treatment-Resistant Human Breast Cancer Cell Subtypes: Convergence on the ROS/SUMO Pathway and Its Effects on xCT Expression and System xc- Activity
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for CM-H2DCFDA (General Oxidative Stress Indicator) below.

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