Protocol tips
Upstream tips |
Protocol tips |
Downstream tips |
- Cells should be healthy and not overcrowded since results of the experiments will depend significantly on the cells’ condition. |
- The cells were stained using the two‐color ROS Detection Kit.
-Cells should be pre-treated with the ROS Inhibitor at least 30 minutes prior to induction. |
|
Upstream tips |
- Cells should be healthy and not overcrowded since results of the experiments will depend significantly on the cells’ condition. |
Protocol tips |
- The cells were stained using the two‐color ROS Detection Kit.
-Cells should be pre-treated with the ROS Inhibitor at least 30 minutes prior to induction. |
Publication protocol
Reactive oxygen species (ROS) were measured using a total ROS/Superoxide Detection Kit (Enzo Life Science, Farmingdale, NY, USA). Using a combination of two specific fluorescent probes, the kit provides a simple and specific assay for the real‐time measurement of global ROS levels, specifically of superoxide, in living cells. The cells were stained using the two‐color ROS Detection Kit and analyzed using FCM (flow cytometry).
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Papers
Check out relevant papers found by Labettor's AI that are relevant for performing ROS assay cell type - PLHC-1, SK-HEP-1, Hep3b, HepG2 human hepatocellular carcinoma using ROS-ID® Total ROS/Superoxide detection kit from Enzo Life Sciences.
Paper title
Micro‐761 is upregulated in hepatocellular carcinoma and regulates tumorigenesis by targeting Mitofusin‐2
Manufacturer protocol
Download the product protocol from Enzo Life Sciences for ROS-ID® Total ROS/Superoxide detection kit below.
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