ROS-ID® Total ROS/Superoxide detection kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- Cells should be healthy and not overcrowded since results of the experiments will depend significantly on the cells’ condition.	- The cells were stained using the two‐color ROS Detection Kit. -Cells should be pre-treated with the ROS Inhibitor at least 30 minutes prior to induction.

Upstream tips
- Cells should be healthy and not overcrowded since results of the experiments will depend significantly on the cells’ condition.
Protocol tips
- The cells were stained using the two‐color ROS Detection Kit. -Cells should be pre-treated with the ROS Inhibitor at least 30 minutes prior to induction.

Publication protocol

Reactive oxygen species (ROS) were measured using a total ROS/Superoxide Detection Kit (Enzo Life Science, Farmingdale, NY, USA). Using a combination of two specific fluorescent probes, the kit provides a simple and specific assay for the real‐time measurement of global ROS levels, specifically of superoxide, in living cells. The cells were stained using the two‐color ROS Detection Kit and analyzed using FCM (flow cytometry).

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Papers

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Manufacturer protocol

Download the product protocol from Enzo Life Sciences for ROS-ID® Total ROS/Superoxide detection kit below.

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Videos

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