OxiSelect™ In Vitro ROS/RNS Assay Kit (Green Fluorescence)

Protocol tips

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- tissue were homogenized in cold PBS solution (20 mg/ml) and centrifuged at 10 000× g for 5 min before the treatment.

Upstream tips
- tissue were homogenized in cold PBS solution (20 mg/ml) and centrifuged at 10 000× g for 5 min before the treatment.

Publication protocol

Total ROS in tumor tissues were measured using the Oxiselect In Vitro ROS/RNS Assay Kit (Cell Biolabs), following the manufacturer's instructions. Briefly, tumor tissues were homogenized in cold PBS solution (20 mg/ml) and centrifuged at 10 000× g for 5 min. The supernatant was collected for the assay. The fluorescence (Ex480 nm/Em530 nM) was measured using the Synergy HT Multi-Mode Microplate Reader (Bio-Tek instruments, Inc).

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Manufacturer protocol

Download the product protocol from Cell Biolabs for OxiSelect™ In Vitro ROS/RNS Assay Kit (Green Fluorescence) below.

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