CellROX™ Deep Red Reagent, for oxidative stress detection

Protocol tips

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	- Cells were treated with 5 μM CellROX® Deep Red Reagent. - Cells were incubated at 37 °C for 30 min

Protocol tips
- Cells were treated with 5 μM CellROX® Deep Red Reagent. - Cells were incubated at 37 °C for 30 min

Publication protocol

The cellular ROS was measured using CellROX® Deep Red Reagents (Invitrogen, Grand Island, NY) following manufacturer’s instructions. Briefly, cells were stained with 5 μM CellROX® Deep Red Reagent and incubated at 37 °C for 30 min. Then, cells were washed with PBS and analyzed on a BD FACS Aria II Flow Cytometer (BD Biosciences, San Jose, CA).

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