OxiSelect™ In Vitro ROS/RNS Assay Kit (Green Fluorescence)

Protocol tips

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	- Cells were incubated with DCFH-DA for 1 h at 37°C. - After incubation with DCFH-DA cells were treated with Tanshinone I and/or TRAIL for 1 h at 37°C

Protocol tips
- Cells were incubated with DCFH-DA for 1 h at 37°C. - After incubation with DCFH-DA cells were treated with Tanshinone I and/or TRAIL for 1 h at 37°C

Publication protocol

PC-3 or DU145 cells were cultured and then pre-incubated with dichloro-dihydro-fluorescein diacetate (DCFH-DA) for 1 h at 37°C. After incubation, DCFH-DA added cells were treated with Tanshinone I and/or TRAIL for 1 h at 37°C. To measure intracellular levels of ROS, the concentration of fluorescent 2',7'-dichlorofluorescein (DCF) was quantified in oxiselect intracellular ROS assay kits (Cell Biolabs, CA, USA) using Fluoroskan Ascent microplate fluorometer (Thomas scientific, NJ, USA).

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Manufacturer protocol

Download the product protocol from Cell Biolabs for OxiSelect™ In Vitro ROS/RNS Assay Kit (Green Fluorescence) below.

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