ROS-ID® Total ROS/Superoxide detection kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- Cells were treated with Oxidative Stress Detection Reagent after treating them with fixatives (4% paraformaldehyde and 100% acetone). - Cells were also immunostained with anti-perilipin antibody and TRITC-conjugated anti-rabbit IgG antibody.

Protocol tips
- Cells were treated with Oxidative Stress Detection Reagent after treating them with fixatives (4% paraformaldehyde and 100% acetone). - Cells were also immunostained with anti-perilipin antibody and TRITC-conjugated anti-rabbit IgG antibody.

Publication protocol

ROS generation from 3T3-L1 cells were assessed using Total ROS Detection kit (Enzo Life Sciences, Plymouth Meeting, PA). Cells were fixed in 4% paraformaldehyde and 100% acetone, and stained with Oxidative Stress Detection Reagent (standard green fluorescence). Cells were also immunostained with anti-perilipin antibody (Cell Signaling Tech., Danvers, MA) and TRITC-conjugated anti-rabbit IgG antibody (Abcam, Cambridge, UK). The cells were mounted with mounting medium containing DAPI and visualized by Olympus FLUOVIEW FV10i confocal microscope (Olympus, Tokyo, Japan).

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing ROS assay cell type - 3T3-L1 using ROS-ID® Total ROS/Superoxide detection kit from Enzo Life Sciences.

Manufacturer protocol

Download the product protocol from Enzo Life Sciences for ROS-ID® Total ROS/Superoxide detection kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing ROS assay cell type - 3T3-L1 using ROS-ID® Total ROS/Superoxide detection kit from Enzo Life Sciences. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.