OxiSelect™ In Vitro ROS/RNS Assay Kit (Green Fluorescence)

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	- Differentiated 3T3-L1 cells were cultured for another 2 days and assayed using ROS kit.

Protocol tips
- Differentiated 3T3-L1 cells were cultured for another 2 days and assayed using ROS kit.

Publication protocol

3T3-L1 preadipocytes were grown to confluence and induced to differentiate into adipocytes, as described previously (21). ROS production was detected using the NBT assay (22). At day 8 after induction, the cells were incubated for 90 min in PBS containing 0.2% NBT. The dark-blue formazan was dissolved in 50% acetic acid, and its absorbance was determined at 570 nm. To monitor ROS production in mature adipocytes, the 3T3-L1 cells differentiated for eight days were further cultured with fresh medium for two days. ROS production was then measured using an OxiSelect Intracellular ROS Assay Kit (Cell Biolabs, Inc., San Diego, CA, USA).

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