Amplex™ Red Hydrogen Peroxide/Peroxidase Assay Kit

Protocol tips

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	- Wells were loaded with Amplex red and exposed to CTX. - For detection of cellular oxidants, cells loaded with dihydroethidium were exposed to CTX for 1 h and assayed.

Protocol tips
- Wells were loaded with Amplex red and exposed to CTX. - For detection of cellular oxidants, cells loaded with dihydroethidium were exposed to CTX for 1 h and assayed.

Publication protocol

Total ROS in C2C12 cells was measured using Amplex® red assay kit (Invitrogen) according to the manufacturer's instructions. Cells in 96-well dishes were loaded with Amplex red and exposed to CTX and the fluorescence corresponding to ROS was recorded in a fluorimeter (Tecan) (excitation = 540 nm; emission = 590 nm). For detection of cellular oxidants, cells loaded with dihydroethidium were exposed to CTX for 1 h, followed by detection of fluorescence in a multiwell fluorimeter (excitation = 518 nm; emission = 606 nm) (22). Quantitation of the higher oxides of nitric oxide (NO) in C2C12 cells was carried out using DAF-FM-DA as described previously (23). Cells loaded with 5 μm DAF-FM-DA were exposed to CTX followed by quantitation of fluorescence (excitation = 495 nm; emission = 515 nm).

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Amplex™ Red Hydrogen Peroxide/Peroxidase Assay Kit below.

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