ROS-ID® Total ROS/Superoxide detection kit

ROS assay cell type - H9c2 rat cardiomyocytes

Experiment
ROS assay cell type - H9c2 rat cardiomyocytes
Product
ROS-ID® Total ROS/Superoxide detection kit from Enzo Life Sciences
Manufacturer
Enzo Life Sciences

Protocol tips

Protocol tips
- Cells were treated with Superoxide Detection Solution and then incubated for 30 min

Publication protocol

To determine the level of superoxide production in cells expressing GFP-tagged GSK-3β, a Total ROS/Superoxide Detection kit (Enzo Life Sciences) was used according to the manufacturer's instructions. Cells were loaded with Superoxide Detection Solution and then incubated for 30 min. After washing the cells twice with wash buffer, the cells were observed by fluorescence microscopy. Fluorescence images of orange fluorescence, indicating superoxide, in GFP-positive cells at a magnification of 400× were analyzed by counting positive pixels per cell by image analysis software (Photoshop CS6, Adobe).

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing ROS assay cell type - H9c2 rat cardiomyocytes using ROS-ID® Total ROS/Superoxide detection kit from Enzo Life Sciences.

Paper title
Translocation of Glycogen Synthase Kinase-3β (GSK-3β), a Trigger of Permeability Transition, Is Kinase Activity-dependent and Mediated by Interaction with Voltage-dependent Anion Channel 2 (VDAC2)
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Manufacturer protocol

Download the product protocol from Enzo Life Sciences for ROS-ID® Total ROS/Superoxide detection kit below.

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