CM-H2DCFDA (General Oxidative Stress Indicator)

ROS assay cell type - PC12

Experiment
ROS assay cell type - PC12
Product
CM-H2DCFDA (General Oxidative Stress Indicator) from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
- Cells were treated with 5 µM CM-H2DCFDA.

- Cells were incubated at 37C for 20 min.

Publication protocol

Cells were seeded at 80% confluency in a 60 mm culture dishes a day prior to drug treatment or transfection. To measure intracellular ROS level, cell pellets were re-suspended in RPMI-1640 medium containing 5 µM of 5-(and-6)-chloromethyl-2-,7-dichlorofluorescin diacetate (CM-H2DCFDA; Molecular Probes, Life Technologies) and incubated in the dark at 37 °C for 20 minutes. Cell suspensions were further diluted and filtered for flow cytometric analysis using BD LSRFortessaTM cell analyzer (BD Biosciences) with excitation and emission spectra of 495 nm and 529 nm respectively.

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Papers

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Paper title
KIF1Bβ increases ROS to mediate apoptosis and reinforces its protein expression through O in a positive feedback mechanism in neuroblastoma
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for CM-H2DCFDA (General Oxidative Stress Indicator) below.

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