CytoSelect™ 24-Well Wound Healing Assay

Protocol tips

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		- Cells are visualised on days 0, 1, 2 and 3 to monitor migration of the cells into the wound field.

Downstream tips
- Cells are visualised on days 0, 1, 2 and 3 to monitor migration of the cells into the wound field.

Publication protocol

Claudin-3 siRNA-transfected and mock transfected control cells were plated at 2.5×105 cells per well into Cytoselect wound-healing assay plates (catalog no. CBA-120; Cell Biolabs San Diego, CA, USA) and grown until 95% confluent. Each well contained an insert that created a defined, rectangular wound field. Following removal of the inserts, the cells were stained (stain included in Cytoselect kit) and photographed on days 0, 1, 2 and 3 to monitor migration of the cells into the wound field. Protein extracts were prepared from the mock- and siRNA-transfected cells (as described previously) on days 0 and 3, and subjected to western blot analysis to assess the degree of claudin-3 suppression.

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Manufacturer protocol

Download the product protocol from Cell Biolabs for CytoSelect™ 24-Well Wound Healing Assay below.

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