Oris™ Universal Cell Migration Assembly Kit, 96 wells

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- At step-14, incubate cells for 16–18 h and at Step 15 stain with 0.5% crystal violet

Protocol tips
- At step-14, incubate cells for 16–18 h and at Step 15 stain with 0.5% crystal violet

Publication protocol

Cell migration assay was conducted by Oris Universal Cell Migration Assembly kit (Platypus Technologies, LCC, Madison, WI, USA) following manufacture’s protocol. Briefly, single cell suspension (5 × 104 cells/well/100 μL) was loaded into stopper loaded wells and incubated overnight to permit cell attachment. To start cell migration, the stoppers was removed, wash wells gently with PBS, added complete cell culture medium and incubate for 16–18 h. Pictures of wells were captured with inverted microscopy after fixation and stain with 0.5% crystal violet (Sigma-Aldrich)/50% EtOH. Data were analyzed with ImageJ software (National Institute of Health, Bethesda, MA, USA).


Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Wound healing assay cell type - human MCF-7 using Oris™ Universal Cell Migration Assembly Kit, 96 wells from Platypus Technologies.

Manufacturer protocol

Download the product protocol from Platypus Technologies for Oris™ Universal Cell Migration Assembly Kit, 96 wells below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Wound healing assay cell type - human MCF-7 using Oris™ Universal Cell Migration Assembly Kit, 96 wells from Platypus Technologies. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.