CytoSelect™ 24-Well Wound Healing Assay

Wound healing assay cell type - human Caco-2

Experiment
Wound healing assay cell type - human Caco-2
Product
CytoSelect™ 24-Well Wound Healing Assay from Cell Biolabs
Manufacturer
Cell Biolabs

Protocol tips

Protocol tips
- After washing at step -7 different stimuli were added (Trx-lps, from S. ratti and T. suis, in concentrations of 3 ng, 30 ng, 300 ng, 1 μg, 10 μg, and 25 μg per 500 μL). As a positive control EGF; 0.5 ng, 5 ng, 10 ng, 15 ng, and 25 ng is added and as a negative control LPS were added.

Publication protocol

To monitor epithelial cell migration of Caco-2 cells and the ability of Trx-lps to improve the wound healing process, we used the CytoSelect 24-Well Wound Healing Assay (Cell Biolabs, Inc.) according to the manufacturer's instructions. By means of the CytoSelect wound healing inserts a 0.9 mm wound field was generated. 500 μL of a Caco-2 cell suspension (containing 0.5 × 106 cells) was added to each well after the inserts had firm contact with the bottom of the wells. After overnight incubation, a monolayer was formed, the inserts were removed, the cells were washed, and the different stimuli were added. We used both Trx-lps, from S. ratti and T. suis, in concentrations of 3 ng, 30 ng, 300 ng, 1 μg, 10 μg, and 25 μg per 500 μL. As a positive control the human epidermal growth factor (EGF; 0.5 ng, 5 ng, 10 ng, 15 ng, and 25 ng) was included in order to get the proper concentration for maximal wound healing effects. As negative control cell media and LPS were added. An inverted digital microscope (EVOS™ FL Thermo Fisher Scientific) by Advanced Microscopy Group was used for observation (4x magnification). The cells were incubated for 4 days, whereby each 24 h a picture was taken and the percent closure was calculated.


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Discussion

Discussion

5 years ago

Author: United States

Which cell lines would you recommend for wound healing assays?

I will be studying some properties of cellulose acetate-PLA blend nanofibers. Which cell lines would you recommend for running wound healing assays effectively?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Wound healing assay cell type - human Caco-2 using CytoSelect™ 24-Well Wound Healing Assay from Cell Biolabs.

Paper title
Multifunctional Thioredoxin-Like Protein from the Gastrointestinal Parasitic Nematodes and Affects Mucosal Homeostasis
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Manufacturer protocol

Download the product protocol from Cell Biolabs for CytoSelect™ 24-Well Wound Healing Assay below.

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