IncuCyte® Cell Migration Kit

Wound healing assay cell type - human MCF-10A

Experiment
Wound healing assay cell type - human MCF-10A
Product
IncuCyte® Cell Migration Kit from Essen Bioscience
Manufacturer
Essen Bioscience

Protocol tips

Protocol tips
- Cells are incubated in DMEM/F12 medium included with 1% Horse serum, 4 ng/ml EGF, 2 ug/ml insulin, 20 ng/ml Cholera Toxin, 0.1 mg/ml Hydrocortisone

Publication protocol

MCF10A cells (3.5 × 104) were seeded into 96-well ImageLock plates for 24 hr in DMEM/F12 medium included with 1% horse serum, 4 ng/ml EGF, 2 μg/ml insulin, 20 ng/ml Cholera Toxin, and 0.1 mg/ml hydrocortisone. Automated 96-well cell migration (scratch wound) on IncuCyte was analyzed using the IncuCyte Cell Migration Kit (Essen BioScience), which comprises a 96-pin wound-making tool (WoundMaker), a Cell Migration Analysis software module, and a starter batch of 96-well ImageLock plates.


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Discussion

Discussion

5 years ago

Author: United States

Which cell lines would you recommend for wound healing assays?

I will be studying some properties of cellulose acetate-PLA blend nanofibers. Which cell lines would you recommend for running wound healing assays effectively?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Wound healing assay cell type - human MCF-10A using IncuCyte® Cell Migration Kit from Essen Bioscience.

Paper title
The Genomic Landscape and Clinical Relevance of A-to-I RNA Editing in Human Cancers
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Manufacturer protocol

Download the product protocol from Essen Bioscience for IncuCyte® Cell Migration Kit below.

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