IncuCyte® Cell Migration Kit

Protocol tips

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	- Cells are incubated in DMEM/F12 medium included with 1% Horse serum, 4 ng/ml EGF, 2 ug/ml insulin, 20 ng/ml Cholera Toxin, 0.1 mg/ml Hydrocortisone

Protocol tips
- Cells are incubated in DMEM/F12 medium included with 1% Horse serum, 4 ng/ml EGF, 2 ug/ml insulin, 20 ng/ml Cholera Toxin, 0.1 mg/ml Hydrocortisone

Publication protocol

MCF10A cells (3.5 × 104) were seeded into 96-well ImageLock plates for 24 hr in DMEM/F12 medium included with 1% horse serum, 4 ng/ml EGF, 2 μg/ml insulin, 20 ng/ml Cholera Toxin, and 0.1 mg/ml hydrocortisone. Automated 96-well cell migration (scratch wound) on IncuCyte was analyzed using the IncuCyte Cell Migration Kit (Essen BioScience), which comprises a 96-pin wound-making tool (WoundMaker), a Cell Migration Analysis software module, and a starter batch of 96-well ImageLock plates.


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Manufacturer protocol

Download the product protocol from Essen Bioscience for IncuCyte® Cell Migration Kit below.

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