Oris™ Cell Migration Assay

Wound healing assay cell type - human MCF-10A

Experiment

Wound healing assay cell type - human MCF-10A

Product

Oris™ Cell Migration Assay from Platypus Technologies

Manufacturer

Platypus Technologies

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Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- Culture media were changed to DMEM/F12 containing EGF (35 ng/ml) after overnight incubation. Proliferation inhibitor mitomycin C (25 ng/ml) was used to exclude influence of cell proliferation

Protocol tips
- Culture media were changed to DMEM/F12 containing EGF (35 ng/ml) after overnight incubation. Proliferation inhibitor mitomycin C (25 ng/ml) was used to exclude influence of cell proliferation

Publication protocol

The wound-healing migration assay was performed using the Oris cell migration kit (Platypus Technologies) according to the manufacturer’s instructions. MCF-10A cell invasion was measured using the BioCoat Matrigel Invasion Chamber (BD Biosciences; 8-μm pore size) according to the manufacturer’s protocol.

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Discussion

5 years ago

Author: United States

Which cell lines would you recommend for wound healing assays?

I will be studying some properties of cellulose acetate-PLA blend nanofibers. Which cell lines would you recommend for running wound healing assays effectively?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Wound healing assay cell type - human MCF-10A using Oris™ Cell Migration Assay from Platypus Technologies.

Paper title

MCRIP1, an ERK substrate, mediates ERK-induced gene silencing during epithelial-mesenchymal transition by regulating the co-repressor CtBP.

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Manufacturer protocol

Download the product protocol from Platypus Technologies for Oris™ Cell Migration Assay below.

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