|Fix cell samples with Fixation solution for 1 h.
Incubate in Permeabilisation solution (0.1% Triton X-100 in PBS) for 2 min on ice.
Label and incubate for 60 min at +37°C in a humidified atmosphere in the dark
|If low labelling is found, reduce fixation time or try 2% buffered paraformaldehyde|
In Situ Cell Death Detection Kit, TMR red from Sigma-Aldrich has not yet been reviewed for this experiment
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3 years ago
Author: Ercole Udinesi
I have left my tumor samples in formalin for an extended period of time (around 10 weeks). Do you think I will be able to use them for TUNEL assay and get results? Thank you for your help in advance
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