CytoSelect™ 24-Well Wound Healing Assay

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- Cells were washed with serum-free media, and cells were treated or not with NGF (200 ng/mL) containing or not inhibitors of α9β1 integrin.	- Cells are pictured at 0 and 24 h

Protocol tips
- Cells were washed with serum-free media, and cells were treated or not with NGF (200 ng/mL) containing or not inhibitors of α9β1 integrin.
Downstream tips
- Cells are pictured at 0 and 24 h

Publication protocol

Wound healing assay was performed using CytoSelect kit (Cell Biolabs). Cells were seeded on 24-well plate containing inserts for artificial wound creation (0.9 mm). The next day, inserts were removed, wells were washed with serum-free media, and cells were treated or not with NGF (200 ng/mL) containing or not inhibitors of α9β1 integrin. For positive control, cells were treated with 2% FBS. Progression of wound closing was monitored by taking phase-contrast pictures at a magnification of 100× at times 0 and 24 h. Images were analyzed using ImageJ software. The percentage of wound closure was calculated after 24 h and normalized to the wound area at time 0

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Manufacturer protocol

Download the product protocol from Cell Biolabs for CytoSelect™ 24-Well Wound Healing Assay below.

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