CytoSelect™ 24-Well Wound Healing Assay

Protocol tips

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	- After wash step, wells were treated with different concentrations of ES, ES-LDP or LDP-ES and incubated at 37C until control wound was completely closed.

Protocol tips
- After wash step, wells were treated with different concentrations of ES, ES-LDP or LDP-ES and incubated at 37C until control wound was completely closed.

Publication protocol

Cell migration was assessed in a wound-healing assay. HMEC or 4T1 Cells at 5x105 cells per well were cultured in 24-wells plate provided by the CytoSelect™ 24-Well Wound Healing Assay Kit and allowed to proliferate to form a confluent monolayer. The linear spacer inserted in the well was removed, which created a regular and defined “wound” within the cell monolayer. Wash wells with media to remove dead cells and debris. Wells were treated with different concentrations of ES, ES-LDP or LDP-ES and further cultured until the control wound was fully closed at 37°C. Cells were fixed and images were captured immediately at 40X magnification from light microscopy and cells that migrated to the scraped area were counted using Image-Pro Plus 6.0 software. Each experiment was performed twice, with triplicate samples.

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Manufacturer protocol

Download the product protocol from Cell Biolabs for CytoSelect™ 24-Well Wound Healing Assay below.

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