CytoSelect™ 24-Well Wound Healing Assay

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- After washing, cells were treated with or without 100 ng/mL of leptin.	- Images are captured by microscope at 0, 3, 6, 9, 12, 18, and 24 hours after the removal of the inserts

Protocol tips
- After washing, cells were treated with or without 100 ng/mL of leptin.
Downstream tips
- Images are captured by microscope at 0, 3, 6, 9, 12, 18, and 24 hours after the removal of the inserts

Publication protocol

The effect of leptin on the migration of human epidermal keratinocytes was analyzed with a CytoSelect Wound Healing Assay kit (Cell Biolabs Inc., San Diego, USA). The assay was performed according to the manufacturer’s instructions. In brief, wound healing inserts were put into 24-well cell culture plates. The cells were added to either side of the insert and incubated overnight to form a monolayer. Inserts were removed generating a 0.9-mm open wound field in the monolayer of cells, and then the cells were treated with or without 100 ng/mL of leptin. Images of wound healing were captured using a phase-contrast microscope at 0, 3, 6, 9, 12, 18, and 24 hours after the removal of the inserts. The area of open wound field was calculated by using ImageJ software

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Manufacturer protocol

Download the product protocol from Cell Biolabs for CytoSelect™ 24-Well Wound Healing Assay below.

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