CytoSelect™ 24-Well Wound Healing Assay

Wound healing assay cell type - human epidermal keratinocytes

Experiment
Wound healing assay cell type - human epidermal keratinocytes
Product
CytoSelect™ 24-Well Wound Healing Assay from Cell Biolabs
Manufacturer
Cell Biolabs

Protocol tips

Protocol tips
- After washing, cells were treated with or without 100 ng/mL of leptin.
Downstream tips
- Images are captured by microscope at 0, 3, 6, 9, 12, 18, and 24 hours after the removal of the inserts

Publication protocol

The effect of leptin on the migration of human epidermal keratinocytes was analyzed with a CytoSelect Wound Healing Assay kit (Cell Biolabs Inc., San Diego, USA). The assay was performed according to the manufacturer’s instructions. In brief, wound healing inserts were put into 24-well cell culture plates. The cells were added to either side of the insert and incubated overnight to form a monolayer. Inserts were removed generating a 0.9-mm open wound field in the monolayer of cells, and then the cells were treated with or without 100 ng/mL of leptin. Images of wound healing were captured using a phase-contrast microscope at 0, 3, 6, 9, 12, 18, and 24 hours after the removal of the inserts. The area of open wound field was calculated by using ImageJ software

Full paper   Login or join for free to view the full paper.

Reviews

CytoSelect™ 24-Well Wound Healing Assay from Cell Biolabs has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Discussion

5 years ago

Author: United States

Which cell lines would you recommend for wound healing assays?

I will be studying some properties of cellulose acetate-PLA blend nanofibers. Which cell lines would you recommend for running wound healing assays effectively?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Wound healing assay cell type - human epidermal keratinocytes using CytoSelect™ 24-Well Wound Healing Assay from Cell Biolabs.

Paper title
Leptin Promotes Wound Healing in the Skin
Full paper
Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Cell Biolabs for CytoSelect™ 24-Well Wound Healing Assay below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Wound healing assay cell type - human epidermal keratinocytes using CytoSelect™ 24-Well Wound Healing Assay from Cell Biolabs. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms