CytoSelect™ 24-Well Wound Healing Assay

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- Cells were treated with various concentrations (0, 0.1, 1, 10 ng/ml) of leptin before creating wound.	- Images are captured at 0, 12, 24, and 36 hours after the removal of the inserts

Protocol tips
- Cells were treated with various concentrations (0, 0.1, 1, 10 ng/ml) of leptin before creating wound.
Downstream tips
- Images are captured at 0, 12, 24, and 36 hours after the removal of the inserts

Publication protocol

The effect of leptin on the migration of RT7 cells was analyzed with a CytoSelect Wound Healing Assay kit (Cell Biolabs Inc., San Diego, USA). The assay was performed according to the manufacturer’s instructions. In brief, RT7 cells were seeded into 24-well plates and cultured overnight. After the cells were treated with various concentrations (0, 0.1, 1, 10 ng/ml) of leptin, the inserts, which were equipped to make a 0.9 mm gap to simulate a wound, were removed from the wells to begin the wound healing assay. Each gap was measured at 0, 12, 24, and 36 hours after the removal of the inserts.

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Manufacturer protocol

Download the product protocol from Cell Biolabs for CytoSelect™ 24-Well Wound Healing Assay below.

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