IncuCyte® Cell Migration Kit

Protocol tips

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		- Cell migration was monitored every 2 h for 2 days.

Downstream tips
- Cell migration was monitored every 2 h for 2 days.

Publication protocol

Migration of model cell lines overexpressing CD271 (A375NGFR/CD271; MeWoPar-NGFR/CD271) or controls (A375GFP; MeWoPar-GFP), as well as fotemustine or vindesine resistant cells (MeWoFote; MeWoVind) was performed with the scratch wound cell migration assay (Essen Bioscience Ltd, Welwyn Garden City, Hertfordshire, UK) following the manufacturer's instructions. Briefly, 30 000 cells of each cell line were seeded 24 h before, yielding in a dense cell layer. Reproducibly wounds were scratched with the Wound Maker, a block with 96 pins. After wounding, the 96-well plate was placed into the Incucyte ZOOM life-cell imaging system (Essen Bioscience Ltd.). Cell migration was monitored every 2 h for 2 days, using a × 10 magnification. Serial pictures were stacked for movie preparation using the ImageJ software (https://imagej.nih.gov/ij/). Statistical analysis was performed by using a two-tailed, paired t-test.

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Manufacturer protocol

Download the product protocol from Essen Bioscience for IncuCyte® Cell Migration Kit below.

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