ApoAlert™ DNA Fragmentation Assay Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
If stored at –20°C, 20X SSC must be warmed to room temperature to ensure that all salts are in solution prior to 10X dilution with deionized H2O. After opening the kit, 20X SSC can be stored at room temperature.	You must separate Coplin jars that have been used with DNase from DNase-free Coplin jars.

Upstream tips
If stored at –20°C, 20X SSC must be warmed to room temperature to ensure that all salts are in solution prior to 10X dilution with deionized H2O. After opening the kit, 20X SSC can be stored at room temperature.
Protocol tips
You must separate Coplin jars that have been used with DNase from DNase-free Coplin jars.

Publication protocol

Apoptotic cell death was measured four hours after the addition of Tet by the deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) method, using ApoAlert DNA fragmentation assay kit (Clontech Europe, France), and according to the manufacturer's protocol. Images were analyzed using a Zeiss immunofluorescence microscope.

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing TUNEL assay cell type - HeLa cells human cervical cancer using ApoAlert™ DNA Fragmentation Assay Kit from Takara Bio Inc.

Manufacturer protocol

Download the product protocol from Takara Bio Inc for ApoAlert™ DNA Fragmentation Assay Kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing TUNEL assay cell type - HeLa cells human cervical cancer using ApoAlert™ DNA Fragmentation Assay Kit from Takara Bio Inc. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.