CytoSelect™ 24-Well Wound Healing Assay

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- Wells are washed with DMEM containing 2% FCS to remove the debris. Cells were treated with different concentrations of tested compounds(0712–1, 0712–2 and 0712–3 at 1/100 and 1/1000 dilutions) for further 24 hours.

Protocol tips
- Wells are washed with DMEM containing 2% FCS to remove the debris. Cells were treated with different concentrations of tested compounds(0712–1, 0712–2 and 0712–3 at 1/100 and 1/1000 dilutions) for further 24 hours.

Publication protocol

The effect of substances 0712–1, 0712–2 and 0712–3 at 1/100 and 1/1000 dilutions on wound closure was investigated with CytoSelect ™Wound Healing Assay Kit (Cell Biolabs, Inc., San Diego, USA). NIH 3T3 fibroblasts (25 × 103/500 μl) in DMEM containing 5% FCS were seeded into 24-wells tissue culture plate containing proprietary treated inserts in the plate wells with their “wound field” aligned in the same direction and incubated for 24 h to allow the cells adhere and reach the 60-80% confluence. After removing the inserts from the wells the medium was carefully aspired and wells were washed with test medium (DMEM containing 2% FCS) to remove dead cells and debris. Finally, the cells were treated with different concentrations of tested compounds for further 24 hours. Migration into the wound field was determined by using manual fixing with cell stain solution according to manufacturer’s instructions. Representative images focused on the center of the wound field were photographed. Microscopic imaging of wound closure was analysed using CellD software [24]. Three sets of experiments in duplicates were performed. The influence of compounds on wound closure was compared to untreated control. As positive control DMEM with 5% FCS was used. Density of cells in wells without created wound area (confluent area) was used as 100% wound closure.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Wound healing assay cell type - mouse NIH 3T3 using CytoSelect™ 24-Well Wound Healing Assay from Cell Biolabs.

Manufacturer protocol

Download the product protocol from Cell Biolabs for CytoSelect™ 24-Well Wound Healing Assay below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Wound healing assay cell type - mouse NIH 3T3 using CytoSelect™ 24-Well Wound Healing Assay from Cell Biolabs. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.