Cell Comb™ Scratch Assay

Wound healing assay cell type - mouse MS1

Experiment
Wound healing assay cell type - mouse MS1
Product
Cell Comb™ Scratch Assay from Merck Millipore
Manufacturer
Merck Millipore

Protocol tips

Protocol tips
- Scratched monolayer is washed with Dulbecco’s phosphate-buffered saline to remove any detached cells.
Downstream tips
- Post-wounding images are captured every 6h and between imaging sessions cells are returned to 37 °C, 5% CO2

Publication protocol

The migration ability of endothelial cells was monitored by a scratch wound assay using the four treatment groups as mentioned previously. Twenty-four hours after transfection a confluent monolayer was observed. Cells were then treated with mitomycin C for 2 h to inhibit cell proliferation and washed extensively. The medium was removed, and cells were wounded by scraping with disposable cell combs (cell comb scratch assay kit, catalog No. 17-10191, Merck Millipore). A “cell-free” area with a width of ∼700 μm was created. Cells were immediately washed with DPBS to remove any detached cells from the wound area. Phase contrast images were captured in multiple fields immediately after scratching the cell monolayer (t = 0), and every 6 h until 24 h post-wounding (t = 24) using a Leica microscope. Cells are returned to 37 °C, 5% CO2 between imaging sessions. The length of the wound filled by the migrating cells were measured using Leica Application Suite X (LAS X) software (Leica Microsystems). The percentage of wound closure was calculated based on the equation ((Li − Lt)/Li) × 100%, where Li is the length of wound at t = 0 h and Lt is the length of wound following t hours of culture.

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Discussion

Discussion

5 years ago

Author: United States

Which cell lines would you recommend for wound healing assays?

I will be studying some properties of cellulose acetate-PLA blend nanofibers. Which cell lines would you recommend for running wound healing assays effectively?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Wound healing assay cell type - mouse MS1 using Cell Comb™ Scratch Assay from Merck Millipore.

Paper title
Nitric-oxide synthase trafficking inducer is a pleiotropic regulator of endothelial cell function and signaling
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Manufacturer protocol

Download the product protocol from Merck Millipore for Cell Comb™ Scratch Assay below.

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