CytoSelect™ 24-Well Wound Healing Assay

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- After cells are washed, weels are filled with with MSC-CM, MIV, MSC-CM-dep MIV, 30% FBS, or serum-free DMEM (DMEM []) and incubated at 37°C for 48 hours.

Protocol tips
- After cells are washed, weels are filled with with MSC-CM, MIV, MSC-CM-dep MIV, 30% FBS, or serum-free DMEM (DMEM []) and incubated at 37°C for 48 hours.

Publication protocol

The migratory properties of Zol rMSCs were examined using the CytoSelect Wound Healing Assay kit (Cell Biolabs, San Diego, CA. USA) according to the manufacturer’s instructions. In brief, cell suspension was added to the well using a plastic insert. The insert was removed from the well after a monolayer of cells had formed, creating a wound gap of 0.9 mm. After washing, the cells were incubated at 37°C for 48 hours with MSC-CM, MIV, MSC-CM-dep MIV, 30% FBS, or serum-free DMEM (DMEM []). The extent of wound closure was determined using a light microscope (CK40; Olympus, Tokyo, Japan).

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Manufacturer protocol

Download the product protocol from Cell Biolabs for CytoSelect™ 24-Well Wound Healing Assay below.

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