SLC29A4

siRNA / miRNA gene silencing Human - PANC-1 hENT1

Experiment
siRNA / miRNA gene silencing Human - PANC-1 hENT1
Product
SLC29A4 from Dharmacon
Manufacturer
Dharmacon

Protocol tips

Upstream tips
Seed 5×10^5 cells/dish
Protocol tips
siRNA concentration - 50 nM

Add INTERFERin® to siRNA duplexes and incubate for 10min at RT after homogenization. 

Replace culture medium from cells after 24h.

Incubate the plate at 37°C for 24 to 72 h. 

Publication protocol

The cells were cultured in 6 cm cell culture dish at a density of 5×105 cells/dish. After washing with PBS, cells were treated with INTERFERin (Polyplus transfection™) containing siRNA against hENT1 (SMARTpool: ON-TARGET plus SLC29A, Dharmacon, Inc., Pittsburgh, PA) or negative siRNA (Silencer Negative Control No. 1 siRNA, AM4635, Invitrogen, Grand Island, NY) at a concentration of 50 nM in Opti-MEM (Invitrogen, Grand Island, NY). After 24 hours transfection, cells were washed twice with PBS and incubated for 3 days.

Full paper   Login or join for free to view the full paper.

Reviews

SLC29A4 from Dharmacon has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing siRNA / miRNA gene silencing Human - PANC-1 hENT1 using SLC29A4 from Dharmacon.

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Dharmacon for SLC29A4 below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing siRNA / miRNA gene silencing Human - PANC-1 hENT1 using SLC29A4 from Dharmacon. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms