SLC29A4

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Seed 5×10^5 cells/dish	siRNA concentration - 50 nM Add INTERFERin® to siRNA duplexes and incubate for 10min at RT after homogenization.  Replace culture medium from cells after 24h.   Incubate the plate at 37°C for 24 to 72 h.

Upstream tips
Seed 5×10^5 cells/dish
Protocol tips
siRNA concentration - 50 nM Add INTERFERin® to siRNA duplexes and incubate for 10min at RT after homogenization.  Replace culture medium from cells after 24h.   Incubate the plate at 37°C for 24 to 72 h.

Publication protocol

The cells were cultured in 6 cm cell culture dish at a density of 5×105 cells/dish. After washing with PBS, cells were treated with INTERFERin (Polyplus transfection™) containing siRNA against hENT1 (SMARTpool: ON-TARGET plus SLC29A, Dharmacon, Inc., Pittsburgh, PA) or negative siRNA (Silencer Negative Control No. 1 siRNA, AM4635, Invitrogen, Grand Island, NY) at a concentration of 50 nM in Opti-MEM (Invitrogen, Grand Island, NY). After 24 hours transfection, cells were washed twice with PBS and incubated for 3 days.

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Manufacturer protocol

Download the product protocol from Dharmacon for SLC29A4 below.

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