Dlx-2 siRNA (h)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Seed 2.5×10^5 cells	siRNA concentartion-50 μM Add diluted siRNA to -HilyMax Transfection Reagent to make a mixture   Incubate the mixture for 15 min at room temperature.  Add mixture to cells and incubate for 24h

Upstream tips
Seed 2.5×10^5 cells
Protocol tips
siRNA concentartion-50 μM Add diluted siRNA to -HilyMax Transfection Reagent to make a mixture   Incubate the mixture for 15 min at room temperature.  Add mixture to cells and incubate for 24h

Publication protocol

The si-RNAs targeting DLX2 and Smad2/3 were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). For transfection, cells were plated and grown to 70–90% confluence. Target si-RNA or negative control si-Ct was then delivered to cells (si-DLX2: 50 μM/2.5×105 cells; si-Smad2/3: 100 μM/2.5×105 cells) using HilyMax transfection reagent according to the manufacturer’s instruction. After incubation for 24 h, the cells were detached and irradiated.

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Manufacturer protocol

Download the product protocol from Santa Cruz Biotechnology for Dlx-2 siRNA (h) below.

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