siGENOME Human PAK1 siRNA

siRNA / miRNA gene silencing Human - HeLa PAK1

Experiment
siRNA / miRNA gene silencing Human - HeLa PAK1
Product
siGENOME Human PAK1 siRNA from Horizon Discovery Ltd.
Manufacturer
Horizon Discovery Ltd.

Protocol tips

Protocol tips
Use siRNAs concentartion at 100 nM.

Add diluted siRNA to diluted
Lipofectamine® RNAiMAX
Reagent (1:1 ratio).

Incubate cells for 48-72 h at 37°C.

Publication protocol

HeLa cells were cultured at 37°C in a humidified 5% CO2 atmosphere in Eagle’s Minimum Essential Medium (EMEM) (Sigma) containing 10% heat-inactivated fetal bovine serum (FBS), 100 U/ml penicillin and 0.1 mg/ml streptomycin. RNA interference-mediated gene knockdown was performed using validated Qiagen HP small-interfering RNAs (siRNAs) for clathrin (SI299880), caveolin1 (SI00299642) and caveolin2 (SI02664389). Dynamin I/II siRNA (sc43736) was from Santa Cruz Biotechnology. PKCδ siRNAs (Llado et al., 2004) were designed and validated as described previously. PAK1 siRNAs specific were purchased from Dharmacon (M-003521-04). Negative-control (NC) siRNAs were purchased from Sigma Aldrich or Dharmacon. HeLa cells were transfected with 100 nM of the indicated siRNAs for 48–72 h using LipofectamineTM RNAiMax transfection reagent (Invitrogen) according to the manufacturer’s protocol. Transfection efficiency and effect were evaluated by Western blotting using the indicated antibodies.

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Papers

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Manufacturer protocol

Download the product protocol from Horizon Discovery Ltd. for siGENOME Human PAK1 siRNA below.

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