Anti-LC3 antibody produced in rabbit

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	Cells were lysed in WB/IP lysis buffer (P0013, Beyotime), all the procedures were following the manufacturer's protocol. Subsequently the cell lysates were boiled in 5X SDS-PAGE loading buffer for 10 min and then resolved by 8% SDS-PAGE and transferred to nitrocellulose membrane. The following antibodies were used in this study: LC3 (Sigma), Beclin 1 (CST), PINK1 (CST), Parkin (CST), GAPDH (Proteintech). Bound antibodies were visualized with the ECL kit (Thermo Scientific).

Protocol tips

Cells were lysed in WB/IP lysis buffer (P0013, Beyotime), all the procedures were following the manufacturer's protocol. Subsequently the cell lysates were boiled in 5X SDS-PAGE loading buffer for 10 min and then resolved by 8% SDS-PAGE and transferred to nitrocellulose membrane. The following antibodies were used in this study: LC3 (Sigma), Beclin 1 (CST), PINK1 (CST), Parkin (CST), GAPDH (Proteintech). Bound antibodies were visualized with the ECL kit (Thermo Scientific).

Publication protocol

Cells were lysed in WB/IP lysis buffer (P0013, Beyotime), all the procedures were following the manufacturer's protocol. Subsequently the cell lysates were boiled in 5X SDS-PAGE loading buffer for 10 min and then resolved by 8% SDS-PAGE and transferred to nitrocellulose membrane. The following antibodies were used in this study: LC3 (Sigma), Beclin 1 (CST), PINK1 (CST), Parkin (CST), GAPDH (Proteintech). Bound antibodies were visualized with the ECL kit (Thermo Scientific).

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