AMPKα1/2 siRNA (h)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	siRNA concentraon-30 pmol/well Add diluted siRNA to diluted Lipofectamine Reagent Incubate for 5 min at RT and add to cells. Incubate cells for 1–3 days at 37°C.

Protocol tips
siRNA concentraon-30 pmol/well Add diluted siRNA to diluted Lipofectamine Reagent Incubate for 5 min at RT and add to cells. Incubate cells for 1–3 days at 37°C.

Publication protocol

One day before transfection, HepG2 cells were placed into 24-well plates. When cell densities reached 50% confluent, they were transfected with siRNA directed against AMPKα1/α2 (sc-45312; Santa Cruz, CA, USA) or scrambled siRNA (sc-37007; Santa Cruz, CA, USA) as a control. Briefly, cells were transfected with 30 pmol/well siRNA via Lipofectamine 2000 Transfection Reagent (11668; Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions.

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Manufacturer protocol

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